Whole Blood Preservation for Clinical Trials

TokuKit

Preserve immune cell populations and functional marker subsets for months.

The three step, centrifuge-free process used at over 40 global trial sites.

Order TokuKit

Collect Blood

Collect blood at clinical site & invert the vacutainer 10x.

Fix Sample

Transfer 2 mL of blood to Buffer 1; incubate at room temp for 15 mins.

Store in Freezer

Transfer 4.8 mL of sample to Buffer 2; store at -80°C or ship on dry ice.

15 Month Stability

Preserve population frequencies and protein expression levels from blood draw for 15 months (and counting).

R > 0.99
Correlation of population frequencies
R = 0.96
Correlation of functional marker subsets
R = 0.95
Correlation of protein expression

Fixed like fresh by mass cytometry

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

R > 0.99
Correlation of population frequencies
R = 0.92
Correlation of functional marker subsets
R = 0.85
Correlation of protein expression

6 Month Stability

Preserve population frequencies and protein expression levels from blood draw for 6 months (and counting).

R > 0.99
Correlation of population frequencies
R = 0.98
Correlation of functional marker subsets
R = 0.86
Correlation of median fluorescence intensity

Fixed like fresh by spectral flow

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

R = 0.97
Correlation of population frequencies
R = 0.91
Correlation of functional marker subsets
R = 0.84
Correlation of median fluorescence intensity

TokuKit Stability Data

Don't have access to a -80°C freezer?

If necessary, TokuKit-fixed samples can be stored at 4°C for up to 14 days before transferring to a -80°C freezer.

Product Specifications

Each TokuKit contains the following:

One 2-mL vacutainer
Two disposable transfer pipettes
One 15-mL red top nalgene bottle with 2.8 mL of Stable Lyse buffer
One 15-mL blue top nalgene bottle with 8 mL of Stable Store buffer
One liquid absorbent pad
One resealable plastic biohazard bag

Product dimensions: 6"x4"x2" in

Whole Blood Preservation for Clinical Trials

TokuKit

Preserve immune cell populations and functional marker subsets for months.

The three step, centrifuge-free process used at over 40 global trial sites.

3-Step Preservation

Collect, Fix, and Store

Collect Blood

Fix Sample

Store in Freezer

TokuKit Stability Data

Mass Cytometry

Preserve population frequencies and protein expression levels from blood draw for 15 months (and counting).

Post-fixation Stability

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

Fresh versus TokuKit-Fixed Specimens

TokuKit Stability Data

Spectral Flow Cytometry

Preserve population frequencies and protein expression levels from blood draw for 6 months (and counting).

Post-fixation Stability

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

Fresh versus TokuKit-Fixed Specimens

TokuKit Stability Data

Don't have access to a -80°C freezer?

TokuKit-Fixed Specimens, Storage at -80°C versus 4°C

TokuKit

Documentation

Product Specifications

TokuKit Processing Instructions

Reagant Safety Data Sheets

Regulatory Documentation

See TokuKit in Action

Check our TokuKit demo

Teiko

TokuKit Stability Data

Mass Cytometry