Whole Blood Preservation for Clinical Trials

TokuKit

Preserve immune cell populations and functional marker subsets for months.

The three step, centrifuge-free process used at over 40 global trial sites.

Order TokuKit

Collect Blood

Collect blood at clinical site & invert the vacutainer 10x.

Fix Sample

Transfer 2 mL of blood to Buffer 1; incubate at room temp for 15 mins.

Store in Freezer

Transfer 4.8 mL of sample to Buffer 2; store at -80°C or ship on dry ice.

TokuKit Stability Data

Mass Cytometry

Preservation data on our 41-Marker Standard Panel

15 Month Stability

Preserve population frequencies and protein expression levels from blood draw for 15 months (and counting).

R > 0.99
Correlation of population frequencies
R = 0.95
Correlation of functional marker subsets
R = 0.93
Correlation of protein expression

Fixed like fresh by mass cytometry

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

R > 0.99
Correlation of population frequencies
R = 0.92
Correlation of functional marker subsets
R = 0.85
Correlation of protein expression

TokuKit Stability Data

Spectral Flow Cytometry

Preservation data with our 25-Marker Standard Panel

1 Month Stability

Preserve population frequencies and protein expression levels from blood draw for 1 month (and counting).

R = 1
Correlation of population frequencies
R = 0.99
Correlation of functional marker subsets
R = 0.91
Correlation of median fluorescence intensity

Fixed like fresh by spectral flow

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

R = 0.97
Correlation of population frequencies
R = 0.91
Correlation of functional marker subsets
R = 0.84
Correlation of median fluorescence intensity

TokuKit

Documentation

Instruction manuals, safety data sheets, and regulatory documentation.

Whole Blood Preservation for Clinical Trials

TokuKit

Preserve immune cell populations and functional marker subsets for months.

The three step, centrifuge-free process used at over 40 global trial sites.

3-Step Preservation

Collect, Fix, and Store

Collect Blood

Fix Sample

Store in Freezer

TokuKit Stability Data

Mass Cytometry

Preserve population frequencies and protein expression levels from blood draw for 15 months (and counting).

Post-fixation Stability: 15 Months

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

Fresh versus TokuKit-Fixed Specimens

TokuKit Stability Data

Spectral Flow Cytometry

Preserve population frequencies and protein expression levels from blood draw for 1 month (and counting).

Post-fixation Stability: 1 Month

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

Fresh versus TokuKit-Fixed Specimens

TokuKit

Documentation

TokuKit Processing Instructions

Reagant Safety Data Sheets

Regulatory Documentation

See TokuKit in Action

Check our TokuKit demo

Teiko