Can I use CytoChex with TokuKit without compromising immune cell frequencies?

Yes!

TokuKit—alone–or with CytoChex preserves immune cell populations for PK/PD and exploratory analyses.

Immune Cell Populations Remain Stable with CytoChex + TokuKit

Compared to the standard TokuKit workflow

97%
Cell populations +/ - 3%
<.05%
Average difference across all cell populations

0%
Populations with differences >3%
100%
Of major immune cell populations (T, B, NK, Monocytes, DCs) within +/- 3%

Beyond TBNK: Functional Markers Remain Stable with CytoChex + TokuKit

Compared to the standard TokuKit workflow

93%

Of functional markers within + / - 6%

<4%

Average difference across all functional markers

5/6

Of functional markers fall within + / - 6%

Rare Populations Preserved with CytoChex + TokuKit

When processing live samples isn't possible, CytoChex + TokuKit prevent degradation of fragile populations like plasmablasts.

Experimental Design

Evaluation of blood samples processed with CytoChex +TokuKit vs. standard TokuKit workflow.

Sample Collection:

Whole blood was collected from 3 healthy donors.

Conditions

Blood tubes:
- Sodium Heparin (control)
- CytoChex (Experimental)

Timeline

- Fresh (Control): Samples incubated in TokuKit for 2 hours were frozen at -80°C.
- Transported (Experimental): Samples incubated in TokuKit tube for 48 hours were frozen at -80°C.

All samples were subsequently thawed and processed on the same run on the same day.

Red Blood Cell Lysis

Since TokuKit contains its own lysis buffer, no additional ACK or BD lysis step was required. Given the nature of CytoChex's fixative, no additional ACK or BD lysis step was required.

Panel Design

A 25-marker spectral flow cytometry panel was applied to assess the immune cell populations visualized in the gating strategy below.

What Current Workflows Deliver, and What They Miss

Workflow	Immediate PK/PD	Exploratory Analysis / Long Term Banking
Sodium Heparin (0 H)
CytoChex (0 H, 48 H)
TokuKit (48 H)
CytoChex + TokuKit (0 H , 48 H)

Why does sample preservation matter for your team?

Degradation distorts patient immune profiles

When preservation methods degrade or introduce artifacts, immune profiles shift, creating results that reflect preservation instability rather than a patient's true biology.

Failed samples waste time and budget

Degraded samples often fail QC, requiring costly redraws, repeat shipments, and delays in data delivery.

Inconsistencies undermine PD insights

Differences in how long samples sit before processing introduce noise, making it harder to detect real pharmacodynamic signals, mechanism of action, or biomarkers of response.

Want to learn more?

Ask us about the results of our TokuKit blood volume study—and what studies with TokuKit are coming next.

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Can I use CytoChex with TokuKit without compromising immune cell frequencies?

Compared to the standard TokuKit workflow

Compared to the standard TokuKit workflow

Rare Populations Preserved with CytoChex + TokuKit

When processing live samples isn't possible, CytoChex + TokuKit prevent degradation of fragile populations like plasmablasts.

Experimental Design

Evaluation of blood samples processed with CytoChex +TokuKit vs. standard TokuKit workflow.

Sample Collection:

Conditions

Timeline

Red Blood Cell Lysis

Panel Design

What Current Workflows Deliver, and What They Miss

Why does sample preservation matter for your team?

Want to learn more?

Ask us about the results of our TokuKit blood volume study—and what studies with TokuKit are coming next.

Teiko