For our 25 marker Cytek PBMC panel, we assessed intra-run precision by taking cryopreserved PBMCs from three (3) healthy donors (PBMC-004, PBMC-005, PBMC-006), splitting each sample into three technical replicates, and processing them all together in the same manner. The process was performed by the same operator, who ran all samples together on the same Cytek® Aurora machine during one run. Variance between replicates was calculated for precision analysis. Acceptance criteria were CV ≤ 20% for ≥95% of all measurable (more than 100 cells median) immune cell populations.
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