Blood Preservation Methods for Cytometry

Feature	Transfix Cytomark	Cyto-Chex BCT Streck	WBFL Kit Cytodelics	TokuKit Teiko	MTS1 100 / PROT 1 Smart Tube
Price per Sample	$18-$25 per tube	$20-30 per tube	$22.95-$23.48 per tube (pricing available for 1 & 2mL)	$100 per kit	$23.54 + base station equipment or $5.10 + no base station
Equipment	No	No	Yes Centrifuge	No	Yes Base station available for purchase.
Stability Window	Up to 2 weeks	2 weeks	Up to 1 year	Up to 15 months	N/A
Storage Temperature	2-8°C up to 14 days 18-25°C up to 4 days	2°C to 30°C	≤-80°C	-80°C	-80°C
Shipping Requirements	Room temperature	Room temperature	Dry ice	Dry ice	Dry ice
Protocol Complexity	Single-tube collection	Single-tube collection	12-steps	3-steps, 15 minute incubation at room temp	3-steps, 15 minute incubation at room temp
mL of Blood Preserved	1.8mL	2-5mL	20-100mL	2mL	1mL
Cytometry Staining	Flow Cytometry Surface Only	Flow Cytometry Surface Only	Flow Cytometry Mass Cytometry Surface Markers Intracellular Markers Cytokines	Flow Cytometry Mass Cytometry Surface Markers Intracellular Markers Cytokines	Flow Cytometry Mass Cytometry Surface Markers Intracellular Markers Cytokines
In-House Validated Markers Internally validated for performance	10	7	125	134	N/A
3rd-Party Validated Markers Cited in external publications	148	N/A	16	N/A	136
Inter- & Intra-Run Validated Markers	N/A	7	N/A	33-41	N/A
Contact Information	Buy TransFix	Buy CytoChex	Buy WBFL	Buy TokuKit	Buy MTS1 100

1. Cytomark

Click here to see if your marker has been validated.

*No information regarding the specific in-house methodologies used to validate markers

2. Streck

Click here to see if your marker has been validated.

*Note that Streck's in-house validation methods were done as part of their FDA 510(k) submission

U.S. Food and Drug Administration. (2008).510(k) substantial equivalence determination: Decision summary for K080552.https://www.accessdata.fda.gov/cdrh_docs/reviews/K080552.pdf

3. Cytodelics

Click here to see if your marker has been validated.

*Note that Cytodelics' in-house testing was done by their internal research team

Pass criteria for validation:

Evaluate staining performance and non-specific binding across all white blood cell populations, not just select subsets
Use complete blood samples to identify potential binding issues that might be missed with simplified samples
Select antibody concentrations that minimize background staining across all cell types, even if it means slightly lower signal intensity

4. Teiko

Click here to see if your marker has been validated.

*Note that Teiko's markers are used in CLIA-validated work flows and tested for intra/inter-run precision

Pass criteria for validation: 1. Performed a titration and 2. An optimal staining concentration selected (based on all or some combination of staining index, arc sin ratio, positive/negative population separation, spillover)

Performed a titration
An optimal staining concentration selected (based on all or some combination of staining index, arc sin ratio, positive/negative population separation, spillover).

5. Smart Tube

Click here to see if your marker has been validated.

*No information regarding in-house validated markers

Blood preservation methods for cytometry

Review of current whole blood preservation methods and their applications in cytometry for clinical trials:

Comparison of blood preservation methods used for cytometry

Blood preservation methods side-by-side to find your perfect solution

Are my markers included?

Check to see if your markers are validated across blood preservation method

1. Cytomark

2. Streck

3. Cytodelics

4. Teiko

5. Smart Tube

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