To account for cases where more or less than 2 mL of whole blood is transferred to TokuKit for preservation, we ran a study evaluating the impact of blood volume inputs—0.5, 1, 2, and 3 mL—on both population frequency and functional subset readouts.

To account for cases where samples might be left on the bench at a clinical trial site and fixed for longer than 15 minutes, we ran a study evaluating the impact of extended fixation—15 minutes, 30 minutes, 1 hour, and 2 hours—on both population frequency and functional subset readouts.

When collecting clinical samples for cytometry, the method you choose to preserve blood samples matters. In this study, we show how commonly used approaches—CytoChex, Transfix, and TokuKit—affect immune cell frequencies and data quality.